Journal: The American Journal of Pathology
Article Title: Murine COVID-19 lethality is characterized by lymphoid depletion associated with suppressed antigen presenting cell functionality
doi: 10.1016/j.ajpath.2023.03.008
Figure Lengend Snippet: MHC and STING expression in the lung and spleen of K18-h ACE2 and CAG-h ACE2 mice at 7 dpi. (A) Representative immunofluorescence (IF) staining images for MHCII (green), SP-C (red; AT2 cells), and nuclei marker DAPI (blue) in the lung of K18-h ACE2 and CAG-h ACE2 mice at 7 dpi. Boxed areas were magnified in the right panel. Scale bar = 50 μm (400x), and 20 μm (1000x). (B) Representative IF staining images for MHCII (green), CD11c (red; DCs), and nuclei marker DAPI (blue) in the lung of K18-h ACE2 and CAG-h ACE2 mice at 7 dpi. Boxed areas were magnified in the right panel. Scale bar = 50 μm (400x), and 20 μm (1000x). (C) The percentage of MHC+ cells in SP-C+ ATII cells of the lung of K18-h ACE2 and CAG-h ACE2 mice. (D) The percentage of MHC+ cells in CD11c+ DCs of the lung of K18-h ACE2 and CAG-h ACE2 mice. (E) Proportion of MHCII+ cell types in the lung of CAG-h ACE2 -FVB mice at 7 dpi. (F) Representative IF staining images for MHCII (green), SP-C (red; AT2 cells), STING (white) and nuclei marker DAPI (blue) in the lung of K18-h ACE2 and CAG-h ACE2 mice at 7 dpi. Boxed areas were magnified in the right panel. Scale bar = 50 μm (400x), and 20 μm (1000x). Student’s t test, *p < 0.05; ***p < 0.001. Error bars indicate mean ± SD.
Article Snippet: Mouse anti-SARS-CoV-2 nucleocapsid (Sino Biological), rabbit anti-CD11c (Cell Signaling Technology), rabbit anti-CD19 (Cell Signaling Technology), rat anti-CD3 (Abcam), rat anti-MHCII (eBioscience), rabbit anti-PDPN (Santa Cruz Biotechnology, Dallas, United States), goat anti-SP-C (Santa Cruz Biotechnology), rabbit anti-CD31 (Abcam), and rabbit anti-TMEM173 (Proteintech, Rosemont, United States) were used as primary antibodies.
Techniques: Expressing, Immunofluorescence, Staining, Marker